The use of CNFA lectin in the diagnosis of precancerous changes in the gastric mucosa in patients with Helicobacter pylori-associated chronic gastritisVernygorodskyi Sergii Viktorovyсh, Sukhan Daryna Serhiyivna, Berezovskyi Artur Mykolaiovych, Panasiuk Volodymyr Ivanovych
Department of Pathological Anatomy, Forensic Medicine and Law, National Pirogov Memorial Medical University, Vinnytsya, Ukraine
International Medicine 2020; 2(1): 37-42 | DOI: 10.5455/im.52981 PDF
Background: This study aims to analyze the features of CNFA (Clitocybe nebularis fungus agglutinin labeled) lectin binding to glycoproteins of the gastric mucosa in patients with Helicobacter pylori-associated chronic gastritis with varying degrees of neoplasia.
Methods: Biopsies were performed during endoscopy, considering the requirements of the modified Sydney system. Biopsy material was obtained from 365 people who applied to a regional medical hospital and processed using conventional histological methods. Carbohydrate determinants were detected in accordance with the protocol of lectin-peroxidase-diaminobenzidine staining. The interpretation of the results of the study was carried out by an examination of four pathologists, independently of each other.
Results: Lectin-histochemical analysis of the distribution of carbohydrate determinants to N-acetylgalactosamine-specific lectin CNFA in Helicobacter pylori (+) (H. pylori) patients with chronic non-atrophic gastritis (CNG) and chronic atrophic gastritis (CAG) has established a moderate and diffuse strong CNFA depression in super nuclear zones of the mucous neck cells with a significant increase in the expression of CNFA (p=0.001) in CAG patients with high-grade of neoplasia. At the same time, in 70% and 95% of patients respectively, the expression of CNFA lectin was increased in places of accumulation of Helicobacter infection compared with 30% of H. pylori patients (p=0.001).
Conclusions:According to the results of our study, CNFA lectin may serve as an additional marker for assessing dysplastic changes in the gastric mucosa in patients with Helicobacter pylori-associated chronic gastritis.
Keywords: gastritis, Helicobacter pylori, lectins, neoplasia